A novel peptide to enhance recombinant BMP-2 production in mammalian cell cultures
نویسندگان
چکیده
Background Due to their osteoinductive properties, recombinant human bone morphogenetic proteins (rhBMPs) have been used successfully for bone regeneration and replacement. However, the yields rhBMPs yields in mammalian expression systems are very low, resulting in their high cost. BMPs are synthesized as a precursor, proBMP, which undergoes enzymatic cleavage by proprotein convertases (PCs) to form the mature BMP [1]. Furin, an enzyme of the PC family, has shown to cleave BMP-4 [2] and BMP-2 (Zhou et al., unpublished data). This study investigated the effect and mechanism of action of polyarginine furin inhibitor, IND-1, on rhBMP-2 production in mammalian cell lines overexpressing rhBMP-2.
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